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In this paper is presented the DESAM Toolbox, a set of Mat- lab functions dedicated to the estimation of widely used spectral models for music signals. Although those models can be used in Music Information Retrieval (MIR) tasks, the core functions of the toolbox do not focus on any specific application. It is rather aimed at providing a range of state-of-the-art signal processing tools that decompose music files according to different signal models, giving rise to different “mid-level” representations. After motivating the need for such a toolbox, this paper of- fers an overview of the overall organization of the toolbox, and describes all available functionalities.